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Valine N-monooxygenase

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Valine N-monooxygenase
Identifiers
EC no.1.14.13.118
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
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NCBIproteins

Valine N-monooxygenase (EC 1.14.13.118, CYP79D1, CYP79D2) is an enzyme with systematic name L-valine,NADPH:oxygen oxidoreductase (N-hydroxylating).[1][2] This enzyme catalyses the following chemical reaction

L-valine + 2 O2 + 2 NADPH + 2 H+ (E)-2-methylpropanal oxime + 2 NADP+ + CO2 + 3 H2O (overall reaction)
(1a) L-valine + O2 + NADPH + H+ N-hydroxy-L-valine + NADP+ + H2O
(1b) N-hydroxy-L-valine + O2 + NADPH + H+ N,N-dihydroxy-L-valine + NADP+ + H2O
(1c) N,N-dihydroxy-L-valine (E)-2-methylpropanal oxime + CO2 + H2O (spontaneous reaction)

Valine N-monooxygenase is a heme-thiolate protein (P-450).

References

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  1. ^ Andersen MD, Busk PK, Svendsen I, Møller BL (January 2000). "Cytochromes P-450 from cassava (Manihot esculenta Crantz) catalyzing the first steps in the biosynthesis of the cyanogenic glucosides linamarin and lotaustralin. Cloning, functional expression in Pichia pastoris, and substrate specificity of the isolated recombinant enzymes". The Journal of Biological Chemistry. 275 (3): 1966–75. doi:10.1074/jbc.275.3.1966. PMID 10636899.
  2. ^ Forslund K, Morant M, Jørgensen B, Olsen CE, Asamizu E, Sato S, Tabata S, Bak S (May 2004). "Biosynthesis of the nitrile glucosides rhodiocyanoside A and D and the cyanogenic glucosides lotaustralin and linamarin in Lotus japonicus". Plant Physiology. 135 (1): 71–84. doi:10.1104/pp.103.038059. PMC 429334. PMID 15122013.
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